Ultrafiltration membranes proved effective as initial step towards enzyme purification and for the determination of activity under sub optimal pH conditions. At pH 7.0 steroids, the activity of an enzyme concentrate was inhibited when 1 and 10 mg 1(^ 1) Cd were added to the assay medium, but the presence of this metal in the growth medium did not reduce activity. One dimensional SDS PAGE revealed only one protein difference between strains grown with or without Cd; a reduction in the staining intensity of a 17 kD band of Calothrix D184..
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